Journal: Scientific Reports

Article Title: Impact of LIN7A silencing on U87 cell invasion and its clinical significance in glioblastoma

doi: 10.1038/s41598-025-91285-5

Figure Lengend Snippet: The detailed information of antibodies used in this study.

Article Snippet: Primary antibody , Anti-human nestin (rabbit polyclonal) , IF 1:200 , ZRB69 , MilliporeSigma.

Techniques:

Journal: PLoS ONE

Article Title: Effect of Calcitonin Gene-Related Peptide on the Neurogenesis of Rat Adipose-Derived Stem Cells In Vitro

doi: 10.1371/journal.pone.0086334

Figure Lengend Snippet: Immuostaining analysis to detect the expressions of various protein markers after neural induction on 7 days. (A) expression of Nestin (red); (B) expression of MAP2 (red); (C) expression of RIP (red); and (D) expression of GAFP (red). Scale bars = 100 µm.

Article Snippet: Following transfer, the membranes were blocked with a solution of 0.1% Tween 20/TBS (TBS/T) containing 5% non-fat milk for 1 h at room temperature and subsequently incubated overnight at 4°C with monoclonal mouse anti-human CGRP (Santa Cruz Biotechnology, Santa Cruz, CA, USA, final dilution 1∶400), Bcl-2 (GeneTex, USA, final dilution 1∶300), Wnt-1, Wnt-3a, Wnt-5a, Wnt-7 and β-catenin (Sigma, St. Louis, MO, USA, final dilution 1∶400) antibodies or rabbit polyclonal anti-human nestin, MAP2, RIP, and GFAP antibodies (Sigma, St. Louis, MO, USA, final dilution 1∶500).

Techniques: Expressing

Journal: Journal of Pain Research

Article Title: Tumor Necrosis Factor-α Regulates the TRPA1 Expression in Human Odontoblast-Like Cells

doi: 10.2147/JPR.S255288

Figure Lengend Snippet: TRPA1 expression in HODs and HOD-like cells. ( A and B ) IHC localization of DSPP and nestin in HODs. ( C ) The expression of TRPA1 in cell bodies and cell processes of HODs. The positive expression of DSPP ( D – F ), nestin ( G – I ) and TRPA1 ( J – L ) in HOD-like cells. P dental pulp, D dentin, ODs odontoblast layer. ( A – C: scale bars=50 μm, D – I: scale bars=100 μm, J – L: scale bars=200 μm). Abbreviations: TRPA1, transient receptor potential ankyrin 1; HOD, human odontoblast; IHC, immunohistochemistry; DSPP, dentin sialophosphoprotein; IF, immunofluorescence.

Article Snippet: Briefly, slices were incubated with the following primary antibodies: mouse polyclonal antibody against human DSPP (1:100; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal antibody against human nestin (1:50; Proteintech Group, Chicago, IL, USA), and rabbit polyclonal antibody against human TRPA1 (1:1000; Santa Cruz Biotech) overnight at 4°C.

Techniques: Expressing, Immunohistochemistry, Immunofluorescence